Flora (voiced by Cindy Robinson) - Mother of Thyme who visits her in the city.
Redbud (voiced by Goodson) - A science teacher at the academy who puts her students at risk, even putting their lives on the line, in order to "test" them.
Grog (voiced by Paden) - A creature that is sighted on the academy campus.
Olive (voiced by Stephanie Sheh) – A catgirl working for the Triumverate.
Wyverna Dretch (voiced by Haviland Stillwell) – A female devil professor who teaches an ethics class at the academy.
Aloe (voiced by Joy Lerner) – Anise's elf lesbian wife.
Anise (voiced by Haviland Stillwell) – Sage's lesbian cousin.
Chicory (voiced by AJ Beckles) – The brother of Rosemary who has lavender hair.
Fennel (voiced by Paden) – The father of Rosemary who has violet hair.
Moss Phlox (voiced by Audu Paden) – A satyr professor who teaches blacksmithing and is a guardian.
Professor Caraway (voiced by Raye Rodriguez) – A human professor who teaches a class on sacred alphabets and is a powerful guardian.
Slime Boy (voiced by Julian Koster) – A human warlock boy and second-year student who loves monsters and is a musician.
Neppy Cat (voiced by Cam Clarke) – A housecat near the academy which had secret powers.
Parnelle (voiced by Barbara Goodson) – The youngest male human student in the academy and a child prodigy.
Professor Harkone (voiced by Anthony Brandon Walker) – A human professor who teaches battle tactics.
The Triad (voiced by Salli Saffioti) – The female human headmasters of the academy.
Mandrake (voiced by AJ Beckles) – A human shapeshifter and warlock.
Snapdragon (voiced by Julia Kaye) – A snarky, awkward human student, and friend of Amaryllis, with red hair who goes to the same academy.
Amaryllis (voiced by Katie McVay) – An entitled mean girl human with purple hair who goes to the same academy.
Lavender (voiced by Liisa Lee) – The mother of Rosemary who has lavender hair and was once a well-known Guardian.
Thyme (voiced by Michelle Deco) – Another member of the group and a magical girl with red hair who is elf, and also one of the protagonists later on.
Parsley (voiced by Amber Romero) – One of the protagonists later on, dwarf, and a magical girl with blond hair.
Sage (voiced by Lauren White) – One of the protagonists, a human magical girl and a witch who has blue hair and uses Old Magic.
Rosemary (voiced by Briana Leon) – A human who is presumably the leader of the group of four girls who has pink hair and wants to become a guardian no matter what.
At the same time, they deal with friends, enemies, and betrayals, as they attempt to protect everything from an unknown threat against the world.
A group of four young girls who attend the High Guardian Academy and are training to become guardians of West city.